Construct: shRNA TRCN0000152996

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014892.2-1422s1c1
DNA Barcode:
GCTCAAGATCAAGAACACATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SCAF8 (22828)
Gene Description:
SR-related CTD associated factor 8
Transcript:
RefSeq NM_014892.2 (NON-CURRENT)
Match location:
Position 1422 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000152996)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCAAGATCAAGAACACATT
Hairpin Sequence:
5'-CCGG-GCTCAAGATCAAGAACACATT-CTCGAG-AATGTGTTCTTGATCTTGAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCAAGATCAAGAACACATTCTCGAGAATGTGTTCTTGATCTTGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCAAGATCAAGAACACATTCTCGAGAATGTGTTCTTGATCTTGAGC-3'

Other clones with same target sequence:

(none)