Construct: shRNA TRCN0000153002

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_031887.2-142s1c1
DNA Barcode:
GTTCAGGATACCGAATCAACA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PMCHL1 (5369)
Gene Description:
pro-melanin concentrating hormone like 1 (pseudogene)
Transcript:
RefSeq NM_031887.2 (NON-CURRENT)
Match location:
Position 142 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000153002)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTTCAGGATACCGAATCAACA
Hairpin Sequence:
5'-CCGG-GTTCAGGATACCGAATCAACA-CTCGAG-TGTTGATTCGGTATCCTGAAC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTTCAGGATACCGAATCAACACTCGAGTGTTGATTCGGTATCCTGAACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTTCAGGATACCGAATCAACACTCGAGTGTTGATTCGGTATCCTGAAC-3'

Other clones with same target sequence:

  1. TRCN0000149442