Construct: shRNA TRCN0000153013

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004294.2-1584s1c1
DNA Barcode:
GAAGTCACATTTCCTGACCTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MTRF1 (9617)
Gene Description:
mitochondrial translation release factor 1
Transcript:
RefSeq NM_004294.2 (NON-CURRENT)
Match location:
Position 1584 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000153013)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAGTCACATTTCCTGACCTA
Hairpin Sequence:
5'-CCGG-GAAGTCACATTTCCTGACCTA-CTCGAG-TAGGTCAGGAAATGTGACTTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAGTCACATTTCCTGACCTACTCGAGTAGGTCAGGAAATGTGACTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAGTCACATTTCCTGACCTACTCGAGTAGGTCAGGAAATGTGACTTC-3'

Other clones with same target sequence:

(none)