Construct: shRNA TRCN0000153208

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025170.2-2203s1c1
DNA Barcode:
CTCTTAGGAATATCCCAGGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PREX2 (80243)
Gene Description:
phosphatidylinositol-3,4,5-trisphosphate dependent Rac exchange factor 2
Transcript:
RefSeq NM_025170.2 (NON-CURRENT)
Match location:
Position 2203 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000153208)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTCTTAGGAATATCCCAGGAT
Hairpin Sequence:
5'-CCGG-CTCTTAGGAATATCCCAGGAT-CTCGAG-ATCCTGGGATATTCCTAAGAG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTCTTAGGAATATCCCAGGATCTCGAGATCCTGGGATATTCCTAAGAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTCTTAGGAATATCCCAGGATCTCGAGATCCTGGGATATTCCTAAGAG-3'

Other clones with same target sequence:

(none)