Construct: shRNA TRCN0000153334

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001010892.1-2521s1c1
DNA Barcode:
GCATAATTGTTCTGAGGGTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RSPH4A (345895)
Gene Description:
radial spoke head component 4A
Transcript:
RefSeq NM_001010892.1 (NON-CURRENT)
Match location:
Position 2521 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000153334)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCATAATTGTTCTGAGGGTTA
Hairpin Sequence:
5'-CCGG-GCATAATTGTTCTGAGGGTTA-CTCGAG-TAACCCTCAGAACAATTATGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCATAATTGTTCTGAGGGTTACTCGAGTAACCCTCAGAACAATTATGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCATAATTGTTCTGAGGGTTACTCGAGTAACCCTCAGAACAATTATGC-3'

Other clones with same target sequence:

(none)