Construct: shRNA TRCN0000153398

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_182795.1-664s1c1
DNA Barcode:
GAAGAGGAAGATGATGAGGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NPM2 (10361)
Gene Description:
nucleophosmin/nucleoplasmin 2
Transcript:
RefSeq NM_182795.1 (CURRENT)
Match location:
Position 664 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000153398)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAGAGGAAGATGATGAGGAT
Hairpin Sequence:
5'-CCGG-GAAGAGGAAGATGATGAGGAT-CTCGAG-ATCCTCATCATCTTCCTCTTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAGAGGAAGATGATGAGGATCTCGAGATCCTCATCATCTTCCTCTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAGAGGAAGATGATGAGGATCTCGAGATCCTCATCATCTTCCTCTTC-3'

Other clones with same target sequence:

(none)