Construct: shRNA TRCN0000153430

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_207352.1-1481s1c1
DNA Barcode:
GACAGTTGATTCTTCGTCCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CYP4V2 (285440)
Gene Description:
cytochrome P450 family 4 subfamily V member 2
Transcript:
RefSeq NM_207352.1 (NON-CURRENT)
Match location:
Position 1481 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000153430)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GACAGTTGATTCTTCGTCCAA
Hairpin Sequence:
5'-CCGG-GACAGTTGATTCTTCGTCCAA-CTCGAG-TTGGACGAAGAATCAACTGTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGACAGTTGATTCTTCGTCCAACTCGAGTTGGACGAAGAATCAACTGTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGACAGTTGATTCTTCGTCCAACTCGAGTTGGACGAAGAATCAACTGTC-3'

Other clones with same target sequence:

(none)