Construct: shRNA TRCN0000153470

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024574.2-1760s1c1
DNA Barcode:
CTCTTCCTGAAGACACAAGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NDNF (79625)
Gene Description:
neuron derived neurotrophic factor
Transcript:
RefSeq NM_024574.2 (NON-CURRENT)
Match location:
Position 1760 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000153470)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTCTTCCTGAAGACACAAGAA
Hairpin Sequence:
5'-CCGG-CTCTTCCTGAAGACACAAGAA-CTCGAG-TTCTTGTGTCTTCAGGAAGAG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTCTTCCTGAAGACACAAGAACTCGAGTTCTTGTGTCTTCAGGAAGAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTCTTCCTGAAGACACAAGAACTCGAGTTCTTGTGTCTTCAGGAAGAG-3'

Other clones with same target sequence:

(none)