Construct: shRNA TRCN0000153496

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015162.3-2390s1c1
DNA Barcode:
CGCCAACAATAGCTGACAATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ACSBG1 (23205)
Gene Description:
acyl-CoA synthetase bubblegum family member 1
Transcript:
RefSeq NM_015162.3 (NON-CURRENT)
Match location:
Position 2390 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000153496)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCCAACAATAGCTGACAATT
Hairpin Sequence:
5'-CCGG-CGCCAACAATAGCTGACAATT-CTCGAG-AATTGTCAGCTATTGTTGGCG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCCAACAATAGCTGACAATTCTCGAGAATTGTCAGCTATTGTTGGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCCAACAATAGCTGACAATTCTCGAGAATTGTCAGCTATTGTTGGCG-3'

Other clones with same target sequence:

(none)