Construct: shRNA TRCN0000153572

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018841.2-1255s1c1
DNA Barcode:
GCTCATGTTCTCTACTGGATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GNG12 (55970)
Gene Description:
G protein subunit gamma 12
Transcript:
RefSeq NM_018841.2 (NON-CURRENT)
Match location:
Position 1255 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000153572)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCATGTTCTCTACTGGATT
Hairpin Sequence:
5'-CCGG-GCTCATGTTCTCTACTGGATT-CTCGAG-AATCCAGTAGAGAACATGAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCATGTTCTCTACTGGATTCTCGAGAATCCAGTAGAGAACATGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCATGTTCTCTACTGGATTCTCGAGAATCCAGTAGAGAACATGAGC-3'

Other clones with same target sequence:

(none)