Construct: shRNA TRCN0000153603

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015215.1-4602s1c1
DNA Barcode:
CCCTTCTTCTAATACCAGCTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CAMTA1 (23261)
Gene Description:
calmodulin binding transcription activator 1
Transcript:
RefSeq NM_015215.1 (NON-CURRENT)
Match location:
Position 4602 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000153603)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTTCTTCTAATACCAGCTT
Hairpin Sequence:
5'-CCGG-CCCTTCTTCTAATACCAGCTT-CTCGAG-AAGCTGGTATTAGAAGAAGGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTTCTTCTAATACCAGCTTCTCGAGAAGCTGGTATTAGAAGAAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTTCTTCTAATACCAGCTTCTCGAGAAGCTGGTATTAGAAGAAGGG-3'

Other clones with same target sequence:

(none)