Construct: shRNA TRCN0000153684

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015315.2-1401s1c1
DNA Barcode:
CAACCTAAAGACACTACCCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LARP1 (23367)
Gene Description:
La ribonucleoprotein 1, translational regulator
Transcript:
RefSeq NM_015315.2 (NON-CURRENT)
Match location:
Position 1401 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000153684)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAACCTAAAGACACTACCCAA
Hairpin Sequence:
5'-CCGG-CAACCTAAAGACACTACCCAA-CTCGAG-TTGGGTAGTGTCTTTAGGTTG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAACCTAAAGACACTACCCAACTCGAGTTGGGTAGTGTCTTTAGGTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAACCTAAAGACACTACCCAACTCGAGTTGGGTAGTGTCTTTAGGTTG-3'

Other clones with same target sequence:

(none)