Construct: shRNA TRCN0000153815

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001015047.1-1563s1c1
DNA Barcode:
CACAGGAGACAAGTGCATTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DDX19-DDX19L (544314)
Gene Description:
DDX19-DDX19L protein
Transcript:
RefSeq NM_001015047.1 (NON-CURRENT)
Match location:
Position 1563 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000153815)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CACAGGAGACAAGTGCATTTA
Hairpin Sequence:
5'-CCGG-CACAGGAGACAAGTGCATTTA-CTCGAG-TAAATGCACTTGTCTCCTGTG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCACAGGAGACAAGTGCATTTACTCGAGTAAATGCACTTGTCTCCTGTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACACAGGAGACAAGTGCATTTACTCGAGTAAATGCACTTGTCTCCTGTG-3'

Other clones with same target sequence:

(none)