Construct: shRNA TRCN0000153830

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024560.2-2483s1c1
DNA Barcode:
CCACTTCCTGAAACCAAGAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ACSS3 (79611)
Gene Description:
acyl-CoA synthetase short chain family member 3
Transcript:
RefSeq NM_024560.2 (NON-CURRENT)
Match location:
Position 2483 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000153830)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACTTCCTGAAACCAAGAAT
Hairpin Sequence:
5'-CCGG-CCACTTCCTGAAACCAAGAAT-CTCGAG-ATTCTTGGTTTCAGGAAGTGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACTTCCTGAAACCAAGAATCTCGAGATTCTTGGTTTCAGGAAGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACTTCCTGAAACCAAGAATCTCGAGATTCTTGGTTTCAGGAAGTGG-3'

Other clones with same target sequence:

  1. TRCN0000343485