Construct: shRNA TRCN0000153874

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015996.1-1484s1c1
DNA Barcode:
CCAGATCTACTTCTGGAACAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SIDT2 (51092)
Gene Description:
SID1 transmembrane family member 2
Transcript:
RefSeq NM_015996.1 (NON-CURRENT)
Match location:
Position 1484 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000153874)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGATCTACTTCTGGAACAT
Hairpin Sequence:
5'-CCGG-CCAGATCTACTTCTGGAACAT-CTCGAG-ATGTTCCAGAAGTAGATCTGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGATCTACTTCTGGAACATCTCGAGATGTTCCAGAAGTAGATCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGATCTACTTCTGGAACATCTCGAGATGTTCCAGAAGTAGATCTGG-3'

Other clones with same target sequence:

(none)