Construct: shRNA TRCN0000154139

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_133375.2-934s1c1
DNA Barcode:
CGGGATTATGTGGTGACATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DIS3L (115752)
Gene Description:
DIS3 like exosome 3'-5' exoribonuclease
Transcript:
RefSeq NM_133375.2 (NON-CURRENT)
Match location:
Position 934 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000154139)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGGATTATGTGGTGACATTT
Hairpin Sequence:
5'-CCGG-CGGGATTATGTGGTGACATTT-CTCGAG-AAATGTCACCACATAATCCCG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGGATTATGTGGTGACATTTCTCGAGAAATGTCACCACATAATCCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGGATTATGTGGTGACATTTCTCGAGAAATGTCACCACATAATCCCG-3'

Other clones with same target sequence:

(none)