Construct: shRNA TRCN0000154147

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001010914.1-798s1c1
DNA Barcode:
CGAGGAAGATTCTGTTTCGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ANKRD36C (400986)
Gene Description:
ankyrin repeat domain 36C
Transcript:
RefSeq NM_001010914.1 (NON-CURRENT)
Match location:
Position 798 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000154147)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGAGGAAGATTCTGTTTCGAT
Hairpin Sequence:
5'-CCGG-CGAGGAAGATTCTGTTTCGAT-CTCGAG-ATCGAAACAGAATCTTCCTCG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGAGGAAGATTCTGTTTCGATCTCGAGATCGAAACAGAATCTTCCTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGAGGAAGATTCTGTTTCGATCTCGAGATCGAAACAGAATCTTCCTCG-3'

Other clones with same target sequence:

(none)