Construct: shRNA TRCN0000154234

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_017643.1-406s1c1
DNA Barcode:
CCTCCTAGAACTATTCAGCAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MBTD1 (54799)
Gene Description:
mbt domain containing 1
Transcript:
RefSeq NM_017643.1 (NON-CURRENT)
Match location:
Position 406 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000154234)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCCTAGAACTATTCAGCAT
Hairpin Sequence:
5'-CCGG-CCTCCTAGAACTATTCAGCAT-CTCGAG-ATGCTGAATAGTTCTAGGAGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCCTAGAACTATTCAGCATCTCGAGATGCTGAATAGTTCTAGGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCCTAGAACTATTCAGCATCTCGAGATGCTGAATAGTTCTAGGAGG-3'

Other clones with same target sequence:

(none)