Construct: shRNA TRCN0000154256

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020381.2-657s1c1
DNA Barcode:
CGTGAACACTTCATGTCAGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PDSS2 (57107)
Gene Description:
decaprenyl diphosphate synthase subunit 2
Transcript:
RefSeq NM_020381.2 (NON-CURRENT)
Match location:
Position 657 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000154256)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTGAACACTTCATGTCAGAA
Hairpin Sequence:
5'-CCGG-CGTGAACACTTCATGTCAGAA-CTCGAG-TTCTGACATGAAGTGTTCACG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTGAACACTTCATGTCAGAACTCGAGTTCTGACATGAAGTGTTCACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTGAACACTTCATGTCAGAACTCGAGTTCTGACATGAAGTGTTCACG-3'

Other clones with same target sequence:

(none)