Construct: shRNA TRCN0000154274

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001474.1-134s1c1
DNA Barcode:
GTACAGCCTCCTGAAATGATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GAGE4 (2576)
Gene Description:
G antigen 4
Transcript:
RefSeq NM_001474.1 (CURRENT)
Match location:
Position 134 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000154274)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTACAGCCTCCTGAAATGATT
Hairpin Sequence:
5'-CCGG-GTACAGCCTCCTGAAATGATT-CTCGAG-AATCATTTCAGGAGGCTGTAC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTACAGCCTCCTGAAATGATTCTCGAGAATCATTTCAGGAGGCTGTACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTACAGCCTCCTGAAATGATTCTCGAGAATCATTTCAGGAGGCTGTAC-3'

Other clones with same target sequence:

  1. TRCN0000161627