Construct: shRNA TRCN0000154313
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_020737.1-2942s1c1
- DNA Barcode:
- CAGTTTCGTCTCCCTGTCAAT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- LRFN2 (57497)
- Gene Description:
- leucine rich repeat and fibronectin type III domain containing 2
- Transcript:
- RefSeq NM_020737.1 (NON-CURRENT)
- Match location:
- Position 2942 (3UTR)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000154313)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- CAGTTTCGTCTCCCTGTCAAT
- Hairpin Sequence:
-
5'-CCGG-CAGTTTCGTCTCCCTGTCAAT-CTCGAG-ATTGACAGGGAGACGAAACTG-TTTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGCAGTTTCGTCTCCCTGTCAATCTCGAGATTGACAGGGAGACGAAACTGTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAACAGTTTCGTCTCCCTGTCAATCTCGAGATTGACAGGGAGACGAAACTG-3'
Other clones with same target sequence:
(none)
