Construct: shRNA TRCN0000154337

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_183058.1-343s1c1
DNA Barcode:
CTACGGCATCTTCCAGATCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LYZL2 (119180)
Gene Description:
lysozyme like 2
Transcript:
RefSeq NM_183058.1 (NON-CURRENT)
Match location:
Position 343 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000154337)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTACGGCATCTTCCAGATCAA
Hairpin Sequence:
5'-CCGG-CTACGGCATCTTCCAGATCAA-CTCGAG-TTGATCTGGAAGATGCCGTAG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTACGGCATCTTCCAGATCAACTCGAGTTGATCTGGAAGATGCCGTAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTACGGCATCTTCCAGATCAACTCGAGTTGATCTGGAAGATGCCGTAG-3'

Other clones with same target sequence:

(none)