Construct: shRNA TRCN0000154369

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015193.3-2147s1c1
DNA Barcode:
CCACAAAGTCTTTGCTGCTGT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ARC (23237)
Gene Description:
activity regulated cytoskeleton associated protein
Transcript:
RefSeq NM_015193.3 (NON-CURRENT)
Match location:
Position 2147 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000154369)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACAAAGTCTTTGCTGCTGT
Hairpin Sequence:
5'-CCGG-CCACAAAGTCTTTGCTGCTGT-CTCGAG-ACAGCAGCAAAGACTTTGTGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACAAAGTCTTTGCTGCTGTCTCGAGACAGCAGCAAAGACTTTGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACAAAGTCTTTGCTGCTGTCTCGAGACAGCAGCAAAGACTTTGTGG-3'

Other clones with same target sequence:

(none)