Construct: shRNA TRCN0000154417

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_153021.3-4540s1c1
DNA Barcode:
GCTCCTGGAATGGATACATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PLB1 (151056)
Gene Description:
phospholipase B1
Transcript:
RefSeq NM_153021.3 (NON-CURRENT)
Match location:
Position 4540 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000154417)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCCTGGAATGGATACATTT
Hairpin Sequence:
5'-CCGG-GCTCCTGGAATGGATACATTT-CTCGAG-AAATGTATCCATTCCAGGAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCCTGGAATGGATACATTTCTCGAGAAATGTATCCATTCCAGGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCCTGGAATGGATACATTTCTCGAGAAATGTATCCATTCCAGGAGC-3'

Other clones with same target sequence:

(none)