Construct: shRNA TRCN0000154435
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_153487.2-6843s1c1
- DNA Barcode:
- GCACTGTAAATGTCTAGGCAT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MDGA1 (266727)
- Gene Description:
- MAM domain containing glycosylphosphatidylinositol anchor 1
- Transcript:
- RefSeq NM_153487.2 (NON-CURRENT)
- Match location:
- Position 6843 (3UTR)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000154435)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GCACTGTAAATGTCTAGGCAT
- Hairpin Sequence:
-
5'-CCGG-GCACTGTAAATGTCTAGGCAT-CTCGAG-ATGCCTAGACATTTACAGTGC-TTTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGCACTGTAAATGTCTAGGCATCTCGAGATGCCTAGACATTTACAGTGCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGCACTGTAAATGTCTAGGCATCTCGAGATGCCTAGACATTTACAGTGC-3'
Other clones with same target sequence:
(none)
