Construct: shRNA TRCN0000154457

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001033523.1-584s1c1
DNA Barcode:
GATGGTGATTGCTCACACCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GUSBP2 (387036)
Gene Description:
GUSB pseudogene 2
Transcript:
RefSeq NM_001033523.1 (NON-CURRENT)
Match location:
Position 584 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000154457)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATGGTGATTGCTCACACCAA
Hairpin Sequence:
5'-CCGG-GATGGTGATTGCTCACACCAA-CTCGAG-TTGGTGTGAGCAATCACCATC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATGGTGATTGCTCACACCAACTCGAGTTGGTGTGAGCAATCACCATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATGGTGATTGCTCACACCAACTCGAGTTGGTGTGAGCAATCACCATC-3'

Other clones with same target sequence:

  1. TRCN0000140710
  2. TRCN0000083211