Construct: shRNA TRCN0000154490

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_173613.1-1463s1c1
DNA Barcode:
GCAGCAAGTAAAGGAGCTACA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GOLGA8IP (283796)
Gene Description:
golgin A8 family member I, pseudogene
Transcript:
RefSeq NM_173613.1 (NON-CURRENT)
Match location:
Position 1463 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000154490)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGCAAGTAAAGGAGCTACA
Hairpin Sequence:
5'-CCGG-GCAGCAAGTAAAGGAGCTACA-CTCGAG-TGTAGCTCCTTTACTTGCTGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGCAAGTAAAGGAGCTACACTCGAGTGTAGCTCCTTTACTTGCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGCAAGTAAAGGAGCTACACTCGAGTGTAGCTCCTTTACTTGCTGC-3'

Other clones with same target sequence:

(none)