Construct: shRNA TRCN0000154541

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_153487.2-2159s1c1
DNA Barcode:
GATCCATGAAGAACGCTACAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MDGA1 (266727)
Gene Description:
MAM domain containing glycosylphosphatidylinositol anchor 1
Transcript:
RefSeq NM_153487.2 (NON-CURRENT)
Match location:
Position 2159 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000154541)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATCCATGAAGAACGCTACAT
Hairpin Sequence:
5'-CCGG-GATCCATGAAGAACGCTACAT-CTCGAG-ATGTAGCGTTCTTCATGGATC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATCCATGAAGAACGCTACATCTCGAGATGTAGCGTTCTTCATGGATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATCCATGAAGAACGCTACATCTCGAGATGTAGCGTTCTTCATGGATC-3'

Other clones with same target sequence:

(none)