Construct: shRNA TRCN0000154583

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_017873.2-865s1c1
DNA Barcode:
GATGATCAACCGCTTCTGCTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ASB6 (140459)
Gene Description:
ankyrin repeat and SOCS box containing 6
Transcript:
RefSeq NM_017873.2 (NON-CURRENT)
Match location:
Position 865 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000154583)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATGATCAACCGCTTCTGCTT
Hairpin Sequence:
5'-CCGG-GATGATCAACCGCTTCTGCTT-CTCGAG-AAGCAGAAGCGGTTGATCATC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATGATCAACCGCTTCTGCTTCTCGAGAAGCAGAAGCGGTTGATCATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATGATCAACCGCTTCTGCTTCTCGAGAAGCAGAAGCGGTTGATCATC-3'

Other clones with same target sequence:

(none)