Construct: shRNA TRCN0000154622

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_152442.2-1012s1c1
DNA Barcode:
GCCACATTAGCTGATGAACAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RAD9B (144715)
Gene Description:
RAD9 checkpoint clamp component B
Transcript:
RefSeq NM_152442.2 (NON-CURRENT)
Match location:
Position 1012 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000154622)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCACATTAGCTGATGAACAA
Hairpin Sequence:
5'-CCGG-GCCACATTAGCTGATGAACAA-CTCGAG-TTGTTCATCAGCTAATGTGGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCACATTAGCTGATGAACAACTCGAGTTGTTCATCAGCTAATGTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCACATTAGCTGATGAACAACTCGAGTTGTTCATCAGCTAATGTGGC-3'

Other clones with same target sequence:

(none)