Construct: shRNA TRCN0000154642

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_173814.3-821s1c1
DNA Barcode:
CCACAGGAAATCCCAAACCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PRTG (283659)
Gene Description:
protogenin
Transcript:
RefSeq NM_173814.3 (NON-CURRENT)
Match location:
Position 821 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000154642)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACAGGAAATCCCAAACCAA
Hairpin Sequence:
5'-CCGG-CCACAGGAAATCCCAAACCAA-CTCGAG-TTGGTTTGGGATTTCCTGTGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACAGGAAATCCCAAACCAACTCGAGTTGGTTTGGGATTTCCTGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACAGGAAATCCCAAACCAACTCGAGTTGGTTTGGGATTTCCTGTGG-3'

Other clones with same target sequence:

(none)