Construct: shRNA TRCN0000154659

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014636.1-5030s1c1
DNA Barcode:
GCAAGCAACATCTGCTCTGTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RALGPS1 (9649)
Gene Description:
Ral GEF with PH domain and SH3 binding motif 1
Transcript:
RefSeq NM_014636.1 (NON-CURRENT)
Match location:
Position 5030 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000154659)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAAGCAACATCTGCTCTGTT
Hairpin Sequence:
5'-CCGG-GCAAGCAACATCTGCTCTGTT-CTCGAG-AACAGAGCAGATGTTGCTTGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAAGCAACATCTGCTCTGTTCTCGAGAACAGAGCAGATGTTGCTTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAAGCAACATCTGCTCTGTTCTCGAGAACAGAGCAGATGTTGCTTGC-3'

Other clones with same target sequence:

(none)