Construct: shRNA TRCN0000154661

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016070.2-459s1c1
DNA Barcode:
GTAGTCACGTTTCCCGGAAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MRPS23 (51649)
Gene Description:
mitochondrial ribosomal protein S23
Transcript:
RefSeq NM_016070.2 (NON-CURRENT)
Match location:
Position 459 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000154661)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTAGTCACGTTTCCCGGAAAT
Hairpin Sequence:
5'-CCGG-GTAGTCACGTTTCCCGGAAAT-CTCGAG-ATTTCCGGGAAACGTGACTAC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTAGTCACGTTTCCCGGAAATCTCGAGATTTCCGGGAAACGTGACTACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTAGTCACGTTTCCCGGAAATCTCGAGATTTCCGGGAAACGTGACTAC-3'

Other clones with same target sequence:

(none)