Construct: shRNA TRCN0000154796

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005998.2-718s1c1
DNA Barcode:
GCGTGGAGTCATGATTAACAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CCT3 (7203)
Gene Description:
chaperonin containing TCP1 subunit 3
Transcript:
RefSeq NM_005998.2 (NON-CURRENT)
Match location:
Position 718 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000154796)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGTGGAGTCATGATTAACAA
Hairpin Sequence:
5'-CCGG-GCGTGGAGTCATGATTAACAA-CTCGAG-TTGTTAATCATGACTCCACGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGTGGAGTCATGATTAACAACTCGAGTTGTTAATCATGACTCCACGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGTGGAGTCATGATTAACAACTCGAGTTGTTAATCATGACTCCACGC-3'

Other clones with same target sequence:

  1. TRCN0000352949