Construct: shRNA TRCN0000154841

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_017888.2-1345s1c1
DNA Barcode:
GATGTGCAGATTGTGGATGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ACSM5 (54988)
Gene Description:
acyl-CoA synthetase medium chain family member 5
Transcript:
RefSeq NM_017888.2 (NON-CURRENT)
Match location:
Position 1345 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000154841)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATGTGCAGATTGTGGATGAT
Hairpin Sequence:
5'-CCGG-GATGTGCAGATTGTGGATGAT-CTCGAG-ATCATCCACAATCTGCACATC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATGTGCAGATTGTGGATGATCTCGAGATCATCCACAATCTGCACATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATGTGCAGATTGTGGATGATCTCGAGATCATCCACAATCTGCACATC-3'

Other clones with same target sequence:

(none)