Construct: shRNA TRCN0000154867

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_032445.1-2427s1c1
DNA Barcode:
GCTGAATCCCTACACCAAGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MEGF11 (84465)
Gene Description:
multiple EGF like domains 11
Transcript:
RefSeq NM_032445.1 (NON-CURRENT)
Match location:
Position 2427 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000154867)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGAATCCCTACACCAAGAT
Hairpin Sequence:
5'-CCGG-GCTGAATCCCTACACCAAGAT-CTCGAG-ATCTTGGTGTAGGGATTCAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGAATCCCTACACCAAGATCTCGAGATCTTGGTGTAGGGATTCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGAATCCCTACACCAAGATCTCGAGATCTTGGTGTAGGGATTCAGC-3'

Other clones with same target sequence:

(none)