Construct: shRNA TRCN0000154901

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005496.2-2402s1c1
DNA Barcode:
GCCACAAGAGTAGCATATCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SMC4 (10051)
Gene Description:
structural maintenance of chromosomes 4
Transcript:
RefSeq NM_005496.2 (NON-CURRENT)
Match location:
Position 2402 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000154901)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCACAAGAGTAGCATATCAA
Hairpin Sequence:
5'-CCGG-GCCACAAGAGTAGCATATCAA-CTCGAG-TTGATATGCTACTCTTGTGGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCACAAGAGTAGCATATCAACTCGAGTTGATATGCTACTCTTGTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCACAAGAGTAGCATATCAACTCGAGTTGATATGCTACTCTTGTGGC-3'

Other clones with same target sequence:

  1. TRCN0000285427