Construct: shRNA TRCN0000154957

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_207375.1-549s1c1
DNA Barcode:
GTCAGCAGGAAGCAGTTAAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CLEC2A (387836)
Gene Description:
C-type lectin domain family 2 member A
Transcript:
RefSeq NM_207375.1 (NON-CURRENT)
Match location:
Position 549 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000154957)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTCAGCAGGAAGCAGTTAAAT
Hairpin Sequence:
5'-CCGG-GTCAGCAGGAAGCAGTTAAAT-CTCGAG-ATTTAACTGCTTCCTGCTGAC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTCAGCAGGAAGCAGTTAAATCTCGAGATTTAACTGCTTCCTGCTGACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTCAGCAGGAAGCAGTTAAATCTCGAGATTTAACTGCTTCCTGCTGAC-3'

Other clones with same target sequence:

(none)