Construct: shRNA TRCN0000155030

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001010925.1-318s1c1
DNA Barcode:
GAGCAGAAGATGCCAGATCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ANKRD19P (138649)
Gene Description:
ankyrin repeat domain 19, pseudogene
Transcript:
RefSeq NM_001010925.1 (NON-CURRENT)
Match location:
Position 318 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000155030)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGCAGAAGATGCCAGATCAA
Hairpin Sequence:
5'-CCGG-GAGCAGAAGATGCCAGATCAA-CTCGAG-TTGATCTGGCATCTTCTGCTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGCAGAAGATGCCAGATCAACTCGAGTTGATCTGGCATCTTCTGCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGCAGAAGATGCCAGATCAACTCGAGTTGATCTGGCATCTTCTGCTC-3'

Other clones with same target sequence:

(none)