Construct: shRNA TRCN0000155031

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001472.1-189s1c1
DNA Barcode:
GAACCAGCAACACCTGAAGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GAGE2C (2574)
Gene Description:
G antigen 2C
Transcript:
RefSeq NM_001472.1 (NON-CURRENT)
Match location:
Position 189 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000155031)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAACCAGCAACACCTGAAGAA
Hairpin Sequence:
5'-CCGG-GAACCAGCAACACCTGAAGAA-CTCGAG-TTCTTCAGGTGTTGCTGGTTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAACCAGCAACACCTGAAGAACTCGAGTTCTTCAGGTGTTGCTGGTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAACCAGCAACACCTGAAGAACTCGAGTTCTTCAGGTGTTGCTGGTTC-3'

Other clones with same target sequence:

  1. TRCN0000137684