Construct: shRNA TRCN0000155065

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005336.2-1714s1c1
DNA Barcode:
GCGGTGCCAACATAAACAGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
HDLBP (3069)
Gene Description:
high density lipoprotein binding protein
Transcript:
RefSeq NM_005336.2 (NON-CURRENT)
Match location:
Position 1714 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000155065)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGGTGCCAACATAAACAGAA
Hairpin Sequence:
5'-CCGG-GCGGTGCCAACATAAACAGAA-CTCGAG-TTCTGTTTATGTTGGCACCGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGGTGCCAACATAAACAGAACTCGAGTTCTGTTTATGTTGGCACCGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGGTGCCAACATAAACAGAACTCGAGTTCTGTTTATGTTGGCACCGC-3'

Other clones with same target sequence:

  1. TRCN0000292471