Construct: shRNA TRCN0000155275

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015550.2-2203s1c1
DNA Barcode:
GCTGGAAGCAAGCCATTTAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
OSBPL3 (26031)
Gene Description:
oxysterol binding protein like 3
Transcript:
RefSeq NM_015550.2 (NON-CURRENT)
Match location:
Position 2203 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000155275)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGGAAGCAAGCCATTTAAT
Hairpin Sequence:
5'-CCGG-GCTGGAAGCAAGCCATTTAAT-CTCGAG-ATTAAATGGCTTGCTTCCAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGGAAGCAAGCCATTTAATCTCGAGATTAAATGGCTTGCTTCCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGGAAGCAAGCCATTTAATCTCGAGATTAAATGGCTTGCTTCCAGC-3'

Other clones with same target sequence:

  1. TRCN0000281980