Construct: shRNA TRCN0000155285

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145293.1-483s1c1
DNA Barcode:
GCAGAAAGGAGATTCTACGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
EEF1DP3 (196549)
Gene Description:
eukaryotic translation elongation factor 1 delta pseudogene 3
Transcript:
RefSeq NM_145293.1 (NON-CURRENT)
Match location:
Position 483 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000155285)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGAAAGGAGATTCTACGAA
Hairpin Sequence:
5'-CCGG-GCAGAAAGGAGATTCTACGAA-CTCGAG-TTCGTAGAATCTCCTTTCTGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGAAAGGAGATTCTACGAACTCGAGTTCGTAGAATCTCCTTTCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGAAAGGAGATTCTACGAACTCGAGTTCGTAGAATCTCCTTTCTGC-3'

Other clones with same target sequence:

(none)