Construct: shRNA TRCN0000155520

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002781.2-314s1c1
DNA Barcode:
GATGGACCTCTACCATTACAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PSG5 (5673)
Gene Description:
pregnancy specific beta-1-glycoprotein 5
Transcript:
RefSeq NM_002781.2 (NON-CURRENT)
Match location:
Position 314 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000155520)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATGGACCTCTACCATTACAT
Hairpin Sequence:
5'-CCGG-GATGGACCTCTACCATTACAT-CTCGAG-ATGTAATGGTAGAGGTCCATC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATGGACCTCTACCATTACATCTCGAGATGTAATGGTAGAGGTCCATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATGGACCTCTACCATTACATCTCGAGATGTAATGGTAGAGGTCCATC-3'

Other clones with same target sequence:

(none)