Construct: shRNA TRCN0000155559

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020151.2-3093s1c1
DNA Barcode:
CCTGGGTTATGTCCAAACTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
STARD7 (56910)
Gene Description:
StAR related lipid transfer domain containing 7
Transcript:
RefSeq NM_020151.2 (NON-CURRENT)
Match location:
Position 3093 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000155559)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGGGTTATGTCCAAACTTA
Hairpin Sequence:
5'-CCGG-CCTGGGTTATGTCCAAACTTA-CTCGAG-TAAGTTTGGACATAACCCAGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGGGTTATGTCCAAACTTACTCGAGTAAGTTTGGACATAACCCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGGGTTATGTCCAAACTTACTCGAGTAAGTTTGGACATAACCCAGG-3'

Other clones with same target sequence:

(none)