Construct: shRNA TRCN0000155576

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_198582.1-3137s1c1
DNA Barcode:
CCTCTCAAGTAGCTGGGATTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
KLHL30 (377007)
Gene Description:
kelch like family member 30
Transcript:
RefSeq NM_198582.1 (NON-CURRENT)
Match location:
Position 3137 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000155576)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCTCAAGTAGCTGGGATTA
Hairpin Sequence:
5'-CCGG-CCTCTCAAGTAGCTGGGATTA-CTCGAG-TAATCCCAGCTACTTGAGAGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCTCAAGTAGCTGGGATTACTCGAGTAATCCCAGCTACTTGAGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCTCAAGTAGCTGGGATTACTCGAGTAATCCCAGCTACTTGAGAGG-3'

Other clones with same target sequence:

  1. TRCN0000165697
  2. TRCN0000060503