Construct: shRNA TRCN0000155583

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005988.2-250s1c1
DNA Barcode:
CCAGTCAAAGTATCCACCGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SPRR2A (6700)
Gene Description:
small proline rich protein 2A
Transcript:
RefSeq NM_005988.2 (NON-CURRENT)
Match location:
Position 250 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000155583)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGTCAAAGTATCCACCGAA
Hairpin Sequence:
5'-CCGG-CCAGTCAAAGTATCCACCGAA-CTCGAG-TTCGGTGGATACTTTGACTGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGTCAAAGTATCCACCGAACTCGAGTTCGGTGGATACTTTGACTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGTCAAAGTATCCACCGAACTCGAGTTCGGTGGATACTTTGACTGG-3'

Other clones with same target sequence:

(none)