Construct: shRNA TRCN0000155622

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_032808.3-2261s1c1
DNA Barcode:
CCGCAAGTTCAACATGAAGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LINGO1 (84894)
Gene Description:
leucine rich repeat and Ig domain containing 1
Transcript:
RefSeq NM_032808.3 (NON-CURRENT)
Match location:
Position 2261 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000155622)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGCAAGTTCAACATGAAGAT
Hairpin Sequence:
5'-CCGG-CCGCAAGTTCAACATGAAGAT-CTCGAG-ATCTTCATGTTGAACTTGCGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGCAAGTTCAACATGAAGATCTCGAGATCTTCATGTTGAACTTGCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGCAAGTTCAACATGAAGATCTCGAGATCTTCATGTTGAACTTGCGG-3'

Other clones with same target sequence:

(none)