Construct: shRNA TRCN0000155671

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_030818.2-496s1c1
DNA Barcode:
CTGTGTCAACTACATCGAGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CCDC130 (81576)
Gene Description:
coiled-coil domain containing 130
Transcript:
RefSeq NM_030818.2 (NON-CURRENT)
Match location:
Position 496 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000155671)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTGTGTCAACTACATCGAGAT
Hairpin Sequence:
5'-CCGG-CTGTGTCAACTACATCGAGAT-CTCGAG-ATCTCGATGTAGTTGACACAG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTGTGTCAACTACATCGAGATCTCGAGATCTCGATGTAGTTGACACAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTGTGTCAACTACATCGAGATCTCGAGATCTCGATGTAGTTGACACAG-3'

Other clones with same target sequence:

(none)