Construct: shRNA TRCN0000155778

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001131.2-961s1c1
DNA Barcode:
CCCATCCTGATACATCCTGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CRISP1 (167)
Gene Description:
cysteine rich secretory protein 1
Transcript:
RefSeq NM_001131.2 (NON-CURRENT)
Match location:
Position 961 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000155778)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCATCCTGATACATCCTGAA
Hairpin Sequence:
5'-CCGG-CCCATCCTGATACATCCTGAA-CTCGAG-TTCAGGATGTATCAGGATGGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCATCCTGATACATCCTGAACTCGAGTTCAGGATGTATCAGGATGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCATCCTGATACATCCTGAACTCGAGTTCAGGATGTATCAGGATGGG-3'

Other clones with same target sequence:

(none)